Key Points of DNA Transcription

ACT 1 - Initiation 

• Transcription factors (TFs) recognize and locates the promoter region (TA-TA Box)
• RNA Polymerase II binds the TFs and creates a transcription initiation complex
• DNA begins to unwind and then stabilized 
• RNA Transcription takes place in the complimentary strand of DNA
• RNA Polymerase II begins to transcribe 

ACT 2 - Elongation 

• RNA grows from 5' to 3'
• RNA Polymerase II adds nucleotides to the 3' end of the growing pre-mRNA
• Pre-mRNA is anti parallel with the Template DNA Strand (Antisense), and it's similar with the Coding DNA strand (Sense)
• Uracil replaces thymine within the pre-mRNA
• The direction of the Transcription goes from upstream towards downstream of the DNA strands

ACT 3 - Termination  

• RNA Transcription stops when it reaches the terminating region (AAAUAAAA) 
• Pre-mRNA turns into mRNA (messenger RNA) by applying a G-cap and poly-A tail 
• Introns of the pre-mRNA are cut by Spliceosome and exon parts are bind together